| 出品公司: | Promega |
|---|---|
| 载体名称: | pNL3.2[NlucP/minP] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | SV40 |
| 载体大小: | 3274 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 带有最小启动子的载体，用于使用不稳定的NanoLuc®荧光素酶测量转录反应元件的活性。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pNL3.2[NlucPminP]载体图谱](/uploads/载体图谱/pNL3.2[NlucPminP]载体图谱.png)
载体简介
载体序列
LOCUS pNL3.2[NlucP minP] 3274 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Vector with a minimal promoter for measuring the activity of
transcriptional response elements using destabilized NanoLuc(R)
luciferase.
ACCESSION JQ513370
VERSION .
KEYWORDS pNL3.2[NlucP minP]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3274)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Saturday, June 6, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3274
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
promoter 77..108
/note="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 141..653
/codon_start=1
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
CDS 657..776
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/note="hPEST"
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
polyA_signal 828..949
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1368..1956)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2156..3016)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 3121..3169
/note="synthetic polyadenylation signal"
misc_feature 3183..3274
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
121 gtactgttgg taaagccacc atggtcttca cactcgaaga tttcgttggg gactggcgac
181 agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc
241 agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc
301 tgaagatcga catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc
361 agatcgaaaa aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc
421 tgcactatgg cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac
481 ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt
541 ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc
601 gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg gcgaattctc
661 acggctttcc gcctgaggtt gaagagcaag ccgccggtac attgcctatg tcctgcgcac
721 aagaaagcgg tatggaccgg cacccagccg cttgtgcttc agctcgcatc aacgtctaag
781 gccgcgactc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga
841 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
901 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
961 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
1021 aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc ttgagagcct
1081 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
1141 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg
1201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
1261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
1321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
1381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
1441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
1501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
1561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
1621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
1681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
1741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
1801 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
1861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
1921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
1981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
2041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
2101 atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc agtggttacc
2161 agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg tccatagtgg
2221 cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca ggccccagcg
2281 cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca atgaaccagc
2341 cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc atccagtcta
2401 tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc cgaagagttg
2461 tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct tcgttcaact
2521 ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga aatgcagtca
2581 gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg
2641 taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc ttttccgtga
2701 ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca agctgctctt
2761 gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa gtgctcatca
2821 tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg agatccagtt
2881 cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc accagcgttt
2941 cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa
3001 aatgttggat gctcatactc gtcctttttc aatattattg aagcatttat cagggttact
3061 agtacgtctc tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc
3121 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
3181 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
3241 cccagtgcaa gtgcaggtgc cagaacattt ctct
//
